CST, 70045S, CD23 (E5P8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD23. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD23 (E5P8Z) Rabbit Monoclonal Antibody (CST #70045) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD23 (E5P8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD23 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD23 protein. Background CD23, also known as FcεRII, is a trimeric type II transmembrane glycoprotein member of the calcium-dependent (C-type) lectin family (1). CD23 is expressed in B and T lymphocytes, monocytes, polymorphonuclear leukocytes, follicular dendritic cells, intestinal epithelial cells, and bone marrow stromal cells (2-8). CD23 was first identified as a low-affinity receptor for IgE and the engagement of membrane bound CD23 leads to suppressed IgE production by B lymphocytes (9). CD23 can be cleaved to form a soluble protein which can drive cytokine release in monocytic cells, nitric oxide (NO) production, and the synthesis of cyclic adenosine 3′5′-monophosphate (cAMP) (10). Soluble CD23 signals through integrins, activating MAPK and NF-κB pathways (11). CD23 is a useful marker in the prognosis of neoplastic disease, is elevated in a variety of autoimmune and inflammatory conditions, and is being investigated as a therapeutic target for IgE-mediated allergy, arthritis, and B cell chronic lymphocytic leukemia (CLL) (12). Alternate Names BLAST-2; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD23; CD23 antigen; CD23A; CLEC4J; Fc epsilon receptor II; Fc fragment of IgE receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc-epsilon-RII; FCE2; FCER2; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; Low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; Lymphocyte IgE receptor Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG