CST, 70109T, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jagged1. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (CST #70109) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 15269 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Jagged1 (D4Y1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Jagged1 protein. Based on sequence analyses, this antibody is not predicted to detect Jagged2 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1131 of human Jagged1 protein. Background Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). Alternate Names AGS; AGS1; AHD; AWS; CD339; DCHE; hJ1; JAG1; jagged 1 (Alagille syndrome); jagged canonical Notch ligand 1; Jagged1; JAGL1; MGC104644; Protein jagged-1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG