CST, 70140S, WWP1 (E1W4V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WWP1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, WWP1 (E1W4V) Rabbit Monoclonal Antibody (CST #70140) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WWP1 (E1W4V) Rabbit Monoclonal Antibody recognizes endogenous levels of total WWP1 protein. This antibody detects non-specific bands of unknown origin above 200 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human WWP1 protein. Background WWP1 (WW domain-containing protein 1, AIP5, Tiul1) is a NEDD4 family ubiquitin E3 ligase (1). The protein regulates many cellular processes, including protein trafficking and degradation, transcription, and signaling. WWP1 ubiquitination activity contributes to the progression of many diseases through polyubiquitination of crucial factors, such as cardiac hypertrophy via the WWP1/DVL2 pathway, Ebola virus egress via the WWP1/eVP40 pathway, and muscle atrophy via the WWP1/KLF15 pathway (2-4). Aberrant high expression of WWP1 is found in many types of tumors, including colorectal cancer, oral cancer, gastric cancer, hepatocellular cancer, and breast cancer (5). The upregulation of WWP1 activity enhances polyubiquitination of tumor suppressors, such as ΔNp63α, PTEN, p53, KLF5, and p27, and ultimately promotes cell proliferation, survival, migration, and invasion in tumorigenesis (6-10). Given the dysregulation and oncogenic functions of WWP1 in these diseases, the protein is considered as a promising therapeutic target. Alternate Names AIP5; atrophin-1 interacting protein 5; Atrophin-1-interacting protein 5; DKFZp434D2111; HECT-type E3 ubiquitin transferase WWP1; hSDRP1; Nedd-4-like ubiquitin-protein ligase; NEDD4-like E3 ubiquitin-protein ligase WWP1; TGIF-interacting ubiquitin ligase 1; Tiul1; WW domain containing E3 ubiquitin protein ligase 1; WW domain-containing protein 1; WWP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG