CST, 70142S, RALY Antibody

Polyclonal Antibody for studying RALY. Validated for Western Blotting. Highly specific and rigorously validated in-house, RALY Antibody (CST #70142) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RALY Antibody recognizes endogenous levels of total RALY protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro163 of human RALY protein. Antibodies are purified by protein A and peptide affinity chromatography. Background RALY is a member of the large family of heterogeneous nuclear ribonucleoproteins (hnRNPs). RALY was initially discovered in lethal yellow mice, a condition where heterozygotes display an all yellow coat, obesity, diabetes, and tumors. In this condition, mRNA to the 5' untranslated region of RALY fuses to the agouti transcript (1,2). RALY binds to U-rich elements in coding and non-coding mRNAs undergoing translation (3). RALY has been implicated in certain cancer types, as it can control transcriptional regulation and splicing of E2F1 and PRMT1 mRNAs (4,5). Alternate Names Autoantigen p542; Heterogeneous nuclear ribonucleoprotein C-like 2; hnRNP associated with lethal yellow protein homolog; hnRNP core protein C-like 2; HNRPCL2; MGC117312; P542; RALY; RALY heterogeneous nuclear ribonucleoprotein; RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow); RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)); RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog); RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow); RNA-binding protein (autoantigenic); RNA-binding protein Raly Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit