CST, 70168T, CD20 (E3N7O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD20 mouse. Validated for WB,IP,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD20 (E3N7O) Rabbit Monoclonal Antibody (CST #70168) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity CD20 (E3N7O) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD20 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD20 protein. Background B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6). Alternate Names AA960661; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; Cd20; CD20 antigen; Ly-4; Ly-44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; Ms4a1; Ms4a2 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 30-50 Source/Isotype: Rabbit IgG