CST, 70180T, LARP1 (D8J4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LARP1. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, LARP1 (D8J4F) Rabbit Monoclonal Antibody (CST #70180) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LARP1 (D8J4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total LARP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LARP1 protein. Background La-related protein 1 (LARP1) is a ubiquitously expressed RNA binding protein that promotes both global and specific mRNA translation in cells (1). LARP1 belongs to the La-related protein family and contains two RNA binding domains, a La motif (LAM), and a neighboring RNA recognition motif-like (RRM-L) domain (1). Research studies indicate that LARP1 acts downstream of mTORC1 to facilitate cell proliferation and growth by promoting global mRNA translation and translation of mRNAs containing a 5'Terminal Oligo-Pyrimidine (5'TOP) motif, which code for translational machinery components (2,3). At the molecular level, LARP1 associates with 5'TOP mRNAs and multiple translation machinery components to positively regulate translation (2,4). Additional studies show that LARP1 expression is upregulated in hepatocellular carcinoma (HCC) patients and that high LARP1 expression in HCC negatively correlates with survival rate (5). Alternate Names KIAA0731; La ribonucleoprotein 1, translational regulator; La ribonucleoprotein domain family member 1; La ribonucleoprotein domain family, member 1; La-related protein 1; Lar1; LARP; LARP1; Lhp1; MGC19556 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG