CST, 70205S, XRN1 Antibody

Polyclonal Antibody for studying XRN1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, XRN1 Antibody (CST #70205) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity XRN1 Antibody recognizes endogenous levels of total XRN1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1696 of human XRN1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background 5'-3' exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5'-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover (1,2). This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins (3). XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3' mRNA fragments generated by RISC cleavage. This process does not require decapping or deadenylation (4). XRN1 plays a significant role in viral RNA degradation (5). As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affects innate immunity and viral replication (6). Alternate Names 5'-3' exoribonuclease 1; DKFZp434P0721; DKFZp686B22225; DKFZp686F19113; FLJ41903; SEP1; Strand-exchange protein 1 homolog; XRN1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 200 SOURCE: Rabbit