CST, 70322SF, ID1 (F2M1J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ID1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, ID1 (F2M1J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #70322) is ready to ship. Product Usage Information This product is the carrier free version of product #23369. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 23369 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ID1 (F2M1J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ID1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human ID1 protein. Background Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain but lack a basic DNA-binding domain. Heterodimerization with bHLH transcription factors, therefore, functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID1 plays important regulatory roles downstream of TGF-β and BMP signaling by inhibiting various transcription factors (3,4). SMAD3 is capable of binding to the ID1 promoter, causing upregulation in response to TGF-β (5). High expression of ID1 is associated with increased cancer growth and migration by inhibiting cell cycle regulators p16, p21, and p27 (6,7). ID1 as a therapeutic target is context dependent. While high expression of ID1 is generally associated with poor prognosis, drug sensitivity can vary greatly depending on tumor type (8-10). Alternate Names BHLHB24; Class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); DNA-binding protein inhibitor ID-1; ID; ID1; inhibitor of differentiation 1; Inhibitor of DNA binding 1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein; inhibitor of DNA binding 1, HLH protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG