CST, 70512S, ZEB1 (E2G6Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZEB1/TCF8. Validated for WB,IP,IHC,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, ZEB1 (E2G6Y) Rabbit Monoclonal Antibody (CST #70512) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 83243 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ZEB1 (E2G6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZEB1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the central region of human ZEB1 protein. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8). Alternate Names AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; MGC133261; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF-8; TCF8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG