CST, 70542T, DCPS (E3A4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DCPS. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DCPS (E3A4Q) Rabbit Monoclonal Antibody (CST #70542) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DCPS (E3A4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DCPS protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu140 of human DCPS protein. Background DCPS is a scavenger pyrophosphate and a member of the histidine triad family, which breaks down the mRNA cap structure during the decay process. During 3'-5' mRNA decay, DCPS hydrolyzes m(7)GTP to generate m7GMP (1,2). DCPS also binds m(7)GDP strongly, which inhibits its activity against m(7)GTP (3). DCPS can shuttle from the nucleus to the cytoplasm and also plays a role in pre-mRNA splicing by affecting the nuclear cap-binding protein CBP20 (4). Additionally, DCPS can contribute to miRNA turnover, a function that is independent of decaying activity and dependent on exonuclease XRN2 (5). mRNA cap analogs that are resistant to DCPS activity have been widely utilized in the study of mRNA decay and stability (6-8). Inhibitors of decapping enzymes have been shown to be effective in spinal muscular atrophy mouse models (9). Alternate Names 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase; ARS; DCPS; DCS-1; DCS1; decapping enzyme, scavenger; Decapping scavenger enzyme; epididymis secretory sperm binding protein; heat shock-like protein 1; HINT-5; Hint-related 7meGMP-directed hydrolase; HINT5; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; homolog of C. elegans 7meGMP-directed hydrolase dcs-1; HSL1; HSPC015; m7GpppX diphosphatase; mRNA decapping enzyme; Scavenger mRNA-decapping enzyme DcpS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 39 Source/Isotype: Rabbit IgG