CST, 70569S, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (Sepharose Bead Conjugate)

Monoclonal Antibody for studying . Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (Sepharose Bead Conjugate) (CST #70569) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (Sepharose Bead Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. This antibody conjugate recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG