CST, 70583T, BMP6 (Precursor Specific) (D2V5Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BMP6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BMP6 (Precursor Specific) (D2V5Z) Rabbit Monoclonal Antibody (CST #70583) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BMP6 (Precursor Specific) (D2V5Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total precursor BMP6 protein. This antibody does not recognize mature BMP6 protein. This antibody also recognizes an unknown protein with a 26 kDa molecular weight. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln173 of human BMP6 protein. Background Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belong to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the C-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4). BMP6 plays a critical role in iron metabolism, ovulation, and aldosterone production (5-7). Recent studies have shown that hypermethylation of the BMP6 gene promoter decreases its expression in several cancer types, which contributes to tumor growth and progression (8-11). Alternate Names BMP-6; BMP6; Bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR-1; VGR1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55-65 Source/Isotype: Rabbit IgG