CST, 70659T, P2X4 Receptor (D9R1H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying P2X4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, P2X4 Receptor (D9R1H) Rabbit Monoclonal Antibody (CST #70659) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity P2X4 Receptor (D9R1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total P2X4 receptor protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro120 of human P2X4 receptor protein. Background P2X purinergic receptors are ATP-gated ion channels involved in physiological processes that include inflammation, afferent sensory signaling, and sympathetic motor nerve activity. Seven different vertebrate genes ( - ) encode for individual receptor protein subunits (1). All P2X subunit proteins share similar protein domain structure, but can differ in overall protein length from 384 to 595 amino acids. Each P2X subunit is composed of amino- and carboxy-terminal intracellular domains, two transmembrane domains, and a large extracellular loop that contains ten evenly spaced cysteines and multiple glycosylation sites (2). P2X receptors are found in a variety of cell types and tissues, including central and peripheral nervous system neurons and glial cells, autonomic and sensory neurons, bone, muscle, and hematopoietic tissues (1). P2X purinoceptor 4 (P2X4) trimers are expressed at the cell surface where they act as ligand-gated ion channels for monovalent and divalent cations (3). P2X4 receptors contribute to regulation of synaptic strength through participation in the formation of long-term potentiation (4,5). Research studies indicate that P2X4 receptor expression may play a role in influencing alcohol-drinking behavior and conferring protection to cardiac myocytes during heart failure (6,7). Additional studies show that microglial P2X4 receptors are upregulated during nerve-injury associated neuropathic pain (8). Alternate Names ATP receptor; ATP-gated cation channel protein; P2RX4; P2X purinoceptor 4; P2X receptor, subunit 4; P2X4; P2X4R; Purinergic receptor; purinergic receptor P2X 4; purinergic receptor P2X, ligand gated ion channel, 4; purinergic receptor P2X, ligand-gated ion channel, 4; purinergic receptor P2X4; purinoceptor P2X4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG