CST, 70802SF, S100A8 (E4F8V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying S100A8 mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, S100A8 (E4F8V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #70802) is ready to ship. Product Usage Information This product is the carrier-free version of product #47310. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 47310 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity S100A8 (E4F8V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total S100A8 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse S100A8 recombinant protein. Background S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9). Alternate Names 60B8A; 60B8Ag; AI323541; B8A; B8Ag; Caga; Calgranulin-A; CFA; CFAg; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-1; CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; Mrp8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100a8; S10A8 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 9 Source/Isotype: Rabbit IgG