CST, 70896S, SLP-76 (D1R1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLP76. Validated for WB,WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, SLP-76 (D1R1A) Rabbit Monoclonal Antibody (CST #70896) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 52812 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SLP-76 (D1R1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLP-76 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro390 of human SLP-76 protein. Background SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adaptor protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline-rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adaptor protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline-rich domain of SLP-76 and the SH3 domain of Itk (3). Furthermore, the proline-rich domain of SLP-76 binds to the SH3 domains of Grb2-like adaptor Gads (3,4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk, and PLCγ1 (1). The expression of SLP-76 is tightly regulated; the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4 CD8 double-positive thymocytes (5). Alternate Names 76 kDa tyrosine phosphoprotein; LCP2; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 76 Source/Isotype: Rabbit IgG