CST, 70948S, Notch2 (D76A6) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying Notch2. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Notch2 (D76A6) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #70948) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Notch2 (D76A6) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2378 of human Notch2 protein. Background Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (5). Alternate Names AGS2; HJCYS; hN2; N2ECD; N2ICD; Neurogenic locus notch homolog protein 2; NOTC2; Notch 2; Notch 2 extracellular truncation; Notch 2 intracellular domain; Notch homolog 2; Notch homolog 2 (Drosophila); notch receptor 2; NOTCH2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit