CST, 7098T, FXR2 (D85D6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FXR2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, FXR2 (D85D6) Rabbit Monoclonal Antibody (CST #7098) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FXR2 (D85D6) Rabbit Monoclonal Antibody recognizes endogenous levels of total FXR2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly477 of human FXR2 protein. Background Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation (1). X-linked FMRP (FMR-1) and its two autosomal homologs, FXR1 and FXR2, are polyribosome-associated RNA-binding proteins that are involved in the pathogenesis of fragile X syndrome (1-3). Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). The homolog of FMRP (dFMRP) associates with Argonaute 2 (Ago2) and Dicer and can coimmunoprecipitate with miRNA and siRNA (5). These results suggest that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). Alternate Names FMR1 autosomal homolog 2; FMR1L2; fragile X mental retardation syndrome related protein 2; Fragile X mental retardation syndrome-related protein 2; fragile X mental retardation, autosomal homolog 2; fragile X-mental retardation 1-like 2; FXR2; FXR2P; RNA-binding protein FXR2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG