CST, 70997S, CPSF4 (E2L2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CPSF4. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CPSF4 (E2L2Z) Rabbit Monoclonal Antibody (CST #70997) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CPSF4 (E2L2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total CPSF4 protein. Non-specific non-nuclear staining has been observed in mouse adipose by immunohistochemistry. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala264 of human CPSF4 protein. Background Cleavage and polyadenylation of pre-mRNA is regulated by a core group of proteins called the cleavage and polyadenylation specificity factors (CPSF) (1,2). The CPSF proteins interact with poly(A) polymerase (PAP) to recognize the AAUAAA sequence motif and add poly(A). CPSF can also interact with downstream cleavage factors to precisely cleave the 3' end of pre-mRNA (2,3). CPSF is brought to 3' ends by the carboxy-terminal domain of the Rpb1 subunit of the RNA Polymerase II complex, where it dissociates and initiates polyadenylation (4). CPSF has been shown to have numerous interactions with viral proteins. The influenza NS1 viral protein binds to CPSF4 to prevent 3' end processing of viral RNAs, inhibiting nuclear export (5). Alternate Names cleavage and polyadenylation specific factor 4; cleavage and polyadenylation specific factor 4, 30kDa; Cleavage and polyadenylation specificity factor 30 kDa subunit; Cleavage and polyadenylation specificity factor subunit 4; cleavage-polyadenylation specificity factor, 30kD; CPSF 30 kDa subunit; CPSF30; CPSF4; NAR; Neb-1; NEB1; No arches homolog; no arches-like zinc finger protein; NS1 effector domain-binding protein 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 26, 28 Source/Isotype: Rabbit IgG