CST, 70998S, DHX9 Antibody

Polyclonal Antibody for studying DDX9. Validated for Western Blotting. Highly specific and rigorously validated in-house, DHX9 Antibody (CST #70998) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DHX9 Antibody recognizes endogenous levels of total DHX9 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala25 of human DHX9 protein. Antibodies are purified by peptide affinity chromatography. Background DExH-box helicase 9 (DHX9), also known as nuclear DNA helicase II or RNA helicase A, is a protein involved in unwinding DNA and RNA by using NTP (1). DHX9 can translocate in the 3' to 5' direction and can utilize all dNTPs or rNTPs, and contributes to pre-mRNA splicing (2,3). Essential for embryo development, DHX9 plays roles in many biological processes, including DNA replication, self-renewal, dosage compensation, and neuronal development (4-7). DHX9 is a critical regulator of abnormal DNA secondary structures (8). It is recruited by TDRD3 to R-loops, where it can unwind and resolve them to prevent DNA damage (9-12). DHX9 plays a central role in the formation and resolution of R-loops (8-11). It also can bind to G-quadruplexes and inverted-repeat Alu elements, suppressing formation of circular RNAs (9,13). BRCA1 is recruited to RNA by DHX9 to promote homologous recombination (14). Furthermore, DHX9 has been shown to be upregulated in various cancer types and can also play a role in viral replication (15-17). Alternate Names ATP-dependent RNA helicase A; DDX9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DEAH-box helicase 9; DExH-box helicase 9; DHX9; leukophysin; LKP; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA; RNA helicase A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit