CST, 71044S, NFKBID (F8U2O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFKBID. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NFKBID (F8U2O) Rabbit Monoclonal Antibody (CST #71044) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NFKBID (F8U2O) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFKBID protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu154 of human NFKBID protein. Background The transcriptional activity of the nuclear factor κB (NF-κB) pathway is tightly regulated in various ways, primarily by post-translational modifications of REL proteins in addition to regulation by atypical members of the IκB protein family (1-4). Atypical IκB proteins of the Bcl-3 subfamily, which include Bcl-3, IκBζ, and NFKB inhibitor delta (NFKBID) (2), are not ubiquitinated and degraded after NF-κB activation; rather, they are highly expressed, acting as transcriptional regulators by binding to NF-κB transcription factors in the nucleus (3,4). Like other IκBs, NFKBID and other atypical IκBs contain ankyrin repeats in their structure and act to modulate the NF-κB pathway. However, unlike typical IκB proteins, they do not actively repress nuclear localization of NF-κB proteins (1-5). NFKBID can change NF-κB-mediated transcription in a few ways: regulation of dimer formation, recruitment of histone-modifying enzymes, or stabilization of NF-κB proteins binding to DNA (5). NFKBID is the smallest member of the Bcl-3 subfamily (6). NFKBID has been shown to interact with several different NF-κB dimers in the nucleus and plays a role in regulatory T cell (Treg) development in the thymus (7). NFKBID is important for IL-2-mediated stimulation of CD4 and CD8 cells (8). Mice deficient for NFKBID or c-REL show similar defects in adaptive and innate immune cell function (9,10). Alternate Names I-kappa-B-delta; IkappaBdelta; IkappaBNS; IkB-delta; IKBD; IKBNS; MGC11314; MGC149503; NF-kappa-B inhibitor delta; NFKB inhibitor delta; NFKBID; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta; Q8NI38; T-cell activation NFKB-like protein; TA-NFKBH Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG