CST, 71107T, GLI3 (E6E2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GLI3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GLI3 (E6E2K) Rabbit Monoclonal Antibody (CST #71107) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GLI3 (E6E2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total GLI3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human GLI3 protein. Background GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5). Initially characterized as a negative regulator of Sonic hedgehog (Shh) signaling (6), follow-up studies described GLI3 as both a transcriptional activator and repressor, similar to GLI2 (7). GLI3 is upregulated in multiple cancers (8,9) but has also been shown to play an anti-cancerous role in acute myeloid leukemia (AML) and medulloblastoma (10,11). Alternate Names ACLS; GCPS; GLI family zinc finger 3; GLI-Kruppel family member GLI3; GLI3; GLI3 C-terminally truncated form; GLI3 form of 190 kDa; GLI3 form of 83 kDa; GLI3 full-length protein; GLI3-190; GLI3-83; GLI3FL; glioma-associated oncogene family zinc finger 3; oncogene GLI3; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV; Transcriptional activator GLI3; Transcriptional repressor GLI3R; Zinc finger protein GLI3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG