CST, 71128S, TET1 (E2J2D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TET1 mouse. Validated for Western Blotting,CUT&RUN. Available in 2 sizes. Highly specific and rigorously validated in-house, TET1 (E2J2D) Rabbit Monoclonal Antibody (CST #71128) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, CUT&RUN Specificity / Sensitivity TET1 (E2J2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TET1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TET1 protein. Background Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3,4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). TET1 is highly expressed in embryonic stem cells and is essential for maintaining stem cell pluripotency through demethylation of the promoter (8). Aberrant TET1 expression has also been implicated in a variety of cancers, including hepatocellular carcinoma, T-cell acute lymphoblastic leukemia (T-ALL), and triple-negative breast cancer (TNBC), among others (9-11). Alternate Names 2510010B09Rik; AA517754; BB001228; CXXC finger 6; CXXC-type zinc finger protein 6; Cxxc6; D10Ertd17; D10Ertd17e; Kiaa1676; LCX; Methylcytosine dioxygenase TET1; mKIAA1676; Ten-eleven translocation 1 gene protein homolog; tet methylcytosine dioxygenase 1; tet oncogene 1; Tet1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG