CST, 71183S, Dectin-1/Clec7a (E3P5W) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying dectin-1 mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Dectin-1/Clec7a (E3P5W) Mouse Chimeric Monoclonal Antibody (CST #71183) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Dectin-1/Clec7a (E3P5W) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total Dectin-1/Clec7a protein. Species Reactivity: Mouse Source / Purification This recombinant chimeric antibody is engineered from Dectin-1/Clec7a (E3P5W) Rabbit mAb #30260 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Dectin-1/Clec7a (E3P5W) Rabbit mAb #30260, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of mouse Dectin-1/Clec7a protein. Background Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9). Alternate Names beta-; Beta-glucan receptor; beta-GR; Bgr; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member a; C-type lectin superfamily member 12; CD369; CLC7A; Clec7a; Clecsf; Clecsf12; DC-associated C-type lectin 1; dectin; Dectin-1; Dectin1; Dendritic cell-associated C-type lectin 1 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a