CST, 71236S, GPAT3 (E5R1H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPAT3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GPAT3 (E5R1H) Rabbit Monoclonal Antibody (CST #71236) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GPAT3 (E5R1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPAT3 protein. Background bands of unknown identity are detected at 20, 70, and 170 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His289 of human GPAT3 protein. Background Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first step in the synthesis of triacylglycerol (TAG) and phospholipids by converting glycerol-3-phosphate to lysophosphatidic acid (LPA) (1). The GPAT family consists of four family members: two mitochondrial proteins (GPAT1 and GPAT2) and two microsomal proteins (GPAT3 and GPAT4). Each can differ in tissue expression, regulation, and physiological and pathophysiological roles (2). The microsomal proteins GPAT3 and GPAT4 were originally designated as AGPAT8 and AGPAT6, respectively. 1-acyl-glycerol-3-phosphate acyltransferase (AGPAT) catalyzes the subsequent reaction converting LPA to phosphatidic acid. The genes were renamed to and upon elucidation of GPAT activity (3,4). Both GPAT3 and GPAT4 are expressed in adipose tissue, but GPAT3 is highly induced during adipocyte differentiation (4). Brown adipose tissue (BAT) preferentially expresses GPAT4 (5). GPAT3 and GPAT4 may also be phosphorylated in response to insulin treatment to increase GPAT activity (6). Accumulation of saturated LPA produced by GPAT4 at the ER-mitochondrial contact site can inhibit autophagy through the abnormal formation of omegasomes, which are precursors to the autophagosome (7). Glycerolipid synthesis by GPAT3 and GPAT4 can also regulate activation of macrophages (8). Alternate Names 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10; 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9; 1-acylglycerol-3-phosphate O-acyltransferase 8; 1-acylglycerol-3-phosphate O-acyltransferase 9; 1-AGP acyltransferase 9; 1-AGPAT 9; Acyl-CoA:glycerol-3-phosphate acyltransferase 3; AGPAT 10; AGPAT10; AGPAT8; AGPAT9; endoplasmic reticulum associated GPAT; Glycerol-3-phosphate acyltransferase 3; GPAT-3; GPAT3; hGPAT3; HMFN0839; LPAAT-theta; Lung cancer metastasis-associated protein 1; Lysophosphatidic acid acyltransferase theta; lysophosphatidic acid acyltransferase, theta; MAG-1; MAG1; MGC11324; testis secretory sperm-binding protein Li 213e Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG