CST, 71257S, ASXL2 (E6Z3X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ASXL2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ASXL2 (E6Z3X) Rabbit Monoclonal Antibody (CST #71257) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ASXL2 (E6Z3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total ASXL2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala640 of human ASXL2 protein. Background Additional sex combs-like protein 2 (ASXL2) is a polycomb-associated protein that interacts with polycomb repressive complex 2 (PRC2), which contains the histone methyltransferase EZH2 and functions to mono-, di-, and tri-methylate histone H3 on lysine 27. These histone marks are associated with transcriptional repression (1). In addition, ASXL2 interacts with BRCA1-associated protein 1 (BAP1), the catalytic subunit of the polycomb repressive deubiquitinase complex (PR-DUB), which functions to de-ubiquitinate histone H2A at lysine 119 and activate transcription (2). ASXL2 functions as a transcriptional regulator of adipogenesis, acting to induce peroxisome proliferator-activated receptor gamma (PPARG) adipocyte differentiation, whereas its paralog ASXL1 functions to inhibit PPARG activity (3). ASXL2 is critical for animal viability and growth, as knockout mice experience partial embryonic lethality, premature death, axial skeletal abnormalities, and reduced bone mineral density (4,5). ASXL2 also plays a role in cancer, where truncation mutations are found in pancreatic, breast, and castration-resistant prostate cancers, albeit infrequently (6-9). is also frequently mutated in acute myeloid leukemia patients with t(8;21)/ chromosomal translocations, and these mutations correlate with a 36% incidence of relapse (10). Alternate Names additional sex combs like 2 (Drosophila); additional sex combs like 2, transcriptional regulator; Additional sex combs-like protein 2; ASXH2; ASXL transcriptional regulator 2; ASXL2; DKFZp686C1968; FLJ10898; KIAA1685; polycomb group protein ASXH2; Putative Polycomb group protein ASXL2; SHAPNS Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 210 Source/Isotype: Rabbit IgG