CST, 71283S, YTHDF2 (E2I2H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying YTHDF2. Validated for WB,IP,IF,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, YTHDF2 (E2I2H) Rabbit Monoclonal Antibody (CST #71283) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity YTHDF2 (E2I2H) Rabbit Monoclonal Antibody recognizes endogenous levels of total YTHDF2 protein. Experiments utilizing overexpression constructs indicated that this antibody does not cross-react with other YTHDF proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly167 of human YTHDF2 protein. Background N -methyladenosine (m A) is an abundant RNA modification that plays an important role in mRNA splicing, processing, and stability. The m A modification is specifically recognized by members of the YT521B homology (YTH) domain-containing family (YTHDF), consisting of YTHDF1, YTHDF2, and YTHDF3. All three members of the YTHDF family are primarily cytosolic proteins that share similar sequence and domain structure, including a conserved C-terminal YTH domain that specifically interacts with m A (1). Despite these similarities, recent studies suggest that YTHDF proteins are involved in distinct regulatory functions with minimal overlap. Specifically, YTHDF1 binding has been reported to promote enhanced mRNA translation, but has no measurable effect on mRNA stability (2). Conversely, YTHDF2 binding appears to promote mRNA degradation, but has minimal effect on translation efficiency (3). The function of YTHDF3 is less clear, but it has been proposed to function as an auxiliary protein for both YTHDF1 and YTHDF2, helping to promote either increased mRNA translation or decay, respectively (4). Additional studies offer a different viewpoint, suggesting that all three YTHDF proteins initiate mRNA degradation (5), or mediate increased mRNA stability and protein expression (6), promoting the idea that these proteins may carry out similar rather than distinct functions. Alternate Names 9430020E02Rik; CAHL; CLL-associated antigen KW-14; HGR8; HGRG8; High-glucose-regulated protein 8; NY-REN-2; Renal carcinoma antigen NY-REN-2; YTH domain family protein 2; YTH domain family, member 2; YTH domain-containing family protein 2; YTH N(6)-methyladenosine RNA binding protein 2; YTH N6-methyladenosine RNA binding protein 2; YTHD2; YTHDF2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG