CST, 71299T, F4/80 (BM8.1) Rat Monoclonal Antibody

Monoclonal Antibody for studying EMR1 mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, F4/80 (BM8.1) Rat Monoclonal Antibody (CST #71299) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. This product is stable for 60 months when stored at -20C. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity F4/80 (BM8.1) Rat Monoclonal Antibody recognizes endogenous levels of total F4/80 protein. This antibody detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. Background F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4). Alternate Names Adgre1; Adhesion G protein-coupled receptor E1; AGRE1; Cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; TM7L; TM7LN3 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2b kappa