CST, 71335S, Phospho-NMDA Receptor 2B (GluN2B) (Ser1303) Antibody

Polyclonal Antibody for studying NMDAR2B (Ser1303) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-NMDA Receptor 2B (GluN2B) (Ser1303) Antibody (CST #71335) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-NMDA Receptor 2B (GluN2B) (Ser1303) Antibody recognizes endogenous levels of NMDA Receptor 2B (GluN2B) protein only when phosphorylated at Ser1303. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1303 of human NMDA Recpetor 2B (GluN2B) protein. Antibodies are purified by peptide affinity chromatography. Background N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na and Ca ions into the cell, and K out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6). Ephrin-B2 binding to its receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336, and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca influx in response to glutamate (7). Phosphorylation of NMDAR2B at Ser1303 has been shown to initiate Ca overload during neuronal excitotoxicity and dephosphorylation at this site may confer neuroprotection (8). Alternate Names EIEE27; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; glutamate ionotropic receptor NMDA type subunit 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; GRIN2B; hNR3; MGC142178; MGC142180; MRD6; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NR2B; NR3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 190 SOURCE: Rabbit