CST, 71368T, LDHA (F3T9U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LDH-A mouse. Validated for WB,WB,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, LDHA (F3T9U) Rabbit Monoclonal Antibody (CST #71368) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LDHA (F3T9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total LDHA protein. This antibody does not cross-react with LDHB or LDHC protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse LDHA protein. Background Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD . When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD , which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. Alternate Names L-lactate dehydrogenase A chain; l7R2; lactate dehydrogenase 1, A chain; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase-A; LDH; LDH muscle subunit; Ldh-; Ldh-1; LDH-A; LDH-M; Ldh1; Ldha; Ldhm; M-LDH; OTTMUSP00000017774 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG