CST, 71397SF, CD31 (PECAM-1) (F8M3S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PECAM-1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD31 (PECAM-1) (F8M3S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #71397) is ready to ship. Product Usage Information This product is the carrier free version of product #95462. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 95462 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD31 (PECAM-1) (F8M3S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD31 (PECAM-1) protein. Non-specific staining was observed of human testis by immunohistochemistry. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD31 (PECAM-1) protein. Background CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). Alternate Names adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; FLJ58394; GPIIA'; PECA1; PECAM-1; PECAM-1, CD31/EndoCAM; PECAM1; platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG