CST, 71414S, ENPP3/CD203c (E5M2W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ENPP3. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ENPP3/CD203c (E5M2W) Rabbit Monoclonal Antibody (CST #71414) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ENPP3/CD203c (E5M2W) Rabbit Monoclonal Antibody recognizes endogenous levels of total ENPP3/CD203c protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ENPP3/CD203c protein. Background Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3/CD203c/PD-Ιbeta) is a type-II transmembrane glycoprotein that contains a large extracellular domain, an α-helical transmembrane segment, and a short cytoplasmic domain containing the N-terminus. ENPP3 has been shown to be overexpressed in colon carcinoma and is thought to play a role in tumor initiation and tumor cell invasiveness (1-3). Within the hematopoietic cell compartment, ENPP3 is a cell surface marker of basophil and mast cell lineages (4,5). Indeed, ENPP3 is overexpressed on transformed mast cells in patients with systemic mastocytosis (6). Recently, ENPP3 has been identified as being highly overexpressed in renal cell carcinoma (RCC) and may have potential as a novel therapeutic target for this disease (7). Alternate Names Alkaline phosphodiesterase I; B10; CD203c; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); E-NPP 3; ectonucleotide pyrophosphatase/phosphodiesterase 3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; ENPP3; gp130RB13-6; MAFP; major acidic fibroblast growth factor stimulated phosphoprotein; NPP3; NPPase; Nucleotide diphosphatase; Nucleotide pyrophosphatase; PD-Ibeta; PDNP3; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase-I beta Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG