CST, 71590S, RNF128 Antibody

Polyclonal Antibody for studying RNF128. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RNF128 Antibody (CST #71590) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RNF128 Antibody recognizes endogenous levels of total RNF128 protein. This antibody detects a 40 kDa band of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RNF128 protein. Antibodies are purified by peptide affinity chromatography. Background RNF128, also known as gene related to anergy in lymphocytes (GRAIL), is a RING finger protein family E3 ubiquitin ligase that participates in T cell anergy induction (1,2). RNF128 is a type I transmembrane protein, made up of a zinc RING finger, coiled-coil, and protease-associated domain (1,2). Expression of RNF128/GRAIL has been shown to inhibit cytokine transcription and CD4 T cell proliferation, as well as regulate naïve T cell tolerance induction and Treg cell function (2-4). Alternate Names E3 ubiquitin-protein ligase RNF128; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; RING finger protein 128; ring finger protein 128, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF128; RN128; RNF128 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 SOURCE: Rabbit