CST, 71632S, Tristetraprolin (D1I3T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TTP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Tristetraprolin (D1I3T) Rabbit Monoclonal Antibody (CST #71632) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tristetraprolin (D1I3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Trisetraprolin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala235 of human tristetraprolin protein. Background Tristetraprolin (TTP), also known as NUP475, G0S24, RNF162A, TIS11, and ZFP36, is a CCCH tandem zinc-finger protein that binds to adenosine and uridine (AU)-rich elements (AREs) within 3'-untranslated regions of mRNA and leads to their rapid degradation (1-6). Expression of TTP is rapidly induced by mitogens and growth factors including insulin, phorbol ester, cytokines, and lipopolysaccharide (LPS). In addition, numerous phosphorylation sites on TTP can regulate its stability, nuclear to cytosolic trafficking, as well as controlling its ARE-binding activity. Many of the target mRNAs for TTP, such as TNF-α, have critical roles in inflammation and cancer (2), and mice deficient in TTP develop a systemic autoimmune inflammatory syndrome along with excessive TNF-α levels (7). Furthermore, suppression of TTP expression has been identified as a negative prognostic indicator for some cancers (8). Alternate Names G0/G1 switch regulatory protein 24; G0S24; GOS24; Growth factor-inducible nuclear protein NUP475; mRNA decay activator protein ZFP36; NUP475; Protein TIS11A; RNF162A; TIS11; TIS11A; tristetraprolin; Tristetraproline; TTP; Zfp-36; ZFP36; ZFP36 ring finger protein; Zinc finger protein 36; Zinc finger protein 36 homolog; zinc finger protein 36, C3H type, homolog; zinc finger protein 36, C3H type, homolog (mouse); zinc finger protein, C3H type, 36 homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40-48 Source/Isotype: Rabbit IgG