CST, 71642S, DPF2/BAF45D (E7N8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DPF2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DPF2/BAF45D (E7N8J) Rabbit Monoclonal Antibody (CST #71642) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DPF2/BAF45D (E7N8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total DPF2/BAF45D protein. This antibody does not cross-react with other DPF/BAF45 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human DPF2/BAF45D protein. Background The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8). DPF2/BAF45D, also known as requiem, is a member of the mammalian SWI/SNF complex that was shown to play a role in non-canonical NF-κB signaling (9). DPF2/BAF45D can interact with and decrease levels of OCT4 protein during differentiation (10). Alternate Names Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; CSS7; D4, zinc and double PHD fingers family 2; double PHD fingers 2; DPF2; MGC10180; Protein requiem; REQ; REQU; requiem; requiem, apoptosis response zinc finger; ubi-d4; UBID4; Zinc finger protein ubi-d4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG