CST, 71645L, G4S Linker (E7O2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Simple Western™,Flow Cytometry (Live). Available in 3 sizes. Highly specific and rigorously validated in-house, G4S Linker (E7O2V) Rabbit Monoclonal Antibody (CST #71645) is ready to ship. Product Usage Information Simple Western™: 1:50 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63670 . Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity G4S Linker (E7O2V) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats. Background The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S) or 20-mer (G4S) within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG