CST, 71655S, EGF Receptor (D1P9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EGFR mouse. Validated for WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, EGF Receptor (D1P9C) Rabbit Monoclonal Antibody (CST #71655) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 88433 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity EGF Receptor (D1P9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse EGF receptor protein. This antibody cross-reacts very weakly with overexpressed human EGF receptor. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse EGF receptor protein. Background The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). Alternate Names 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; EGF receptor; Egfr; Epidermal growth factor receptor; Erb; Erbb; Err; Errb1; Errp; OTTMUSP00000005385; OTTMUSP00000005386; wa-2; wa2; Wa5; waved 2 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 175 Source/Isotype: Rabbit IgG