CST, 71692S, GOT2 Antibody

Polyclonal Antibody for studying GOT2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GOT2 Antibody (CST #71692) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GOT2 Antibody recognizes endogenous levels of total GOT2 protein. This antibody does not cross-react with GOT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp54 of human GOT2 protein. Antibodies are purified by peptide affinity chromatography. Background Glutamate oxaloacetate transaminase 2 (GOT2), a mitochondrial enzyme, catalyzes the conversion of oxaloacetate to aspartate. Studies show that the metabolite pyridoxal phosphate (PLP) is selectively required by acute myeloid leukemia. PLP-dependent GOT2 selectively sustains leukemic cell proliferation. Depletion of GOT2 suppresses the proliferation of acute myeloid leukemia cells (1). In addition, mouse hematopoietic stem cells are dependent on cell-autonomous aspartate synthesis. Got2 deletion leads to decreased aspartate levels and reduced hematopoietic stem cell function (2). Furthermore, GOT2-dependent aspartate synthesis is also necessary for protein and collagen biosynthesis in chondrocytes and is critical for chondrocyte proliferation and growth (3). Alternate Names AATM; aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; aspartate transaminase 2; FABP-1; FABPpm; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; glutamic-oxaloacetic transaminase 2; glutamic-oxaloacetic transaminase 2, mitochondrial; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); GOT2; KAT4; KATIV; KYAT4; Kynurenine aminotransferase 4; kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; mAspAT; mitAAT; Plasma membrane-associated fatty acid-binding protein; Transaminase A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 41 SOURCE: Rabbit