CST, 71746S, MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MBOAT2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody (CST #71746) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MBOAT2 (F8Z4U) Rabbit mAb recognizes endogenous levels of total MBOAT2 protein. This antibody does not cross-react with MBOAT1 protein. Non-specific mitotic staining may be observed by immunofluorescence. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MBOAT2 protein. Background Membrane-bound glycerophospholipid O-acyltransferase 2 (MBOAT2) is a phospholipid-modifying enzyme. It transfers an acyl group in an acyl-CoA to a lysophospholipid to produce a phospholipid (1,2). Studies show that MBOAT2 is a ferroptosis suppressor, and this function is independent of GPX4 or AIFM2/FSP1. MBOAT2 selectively catalyzes the transfer of monounsaturated fatty acids (MUFAs) to lysophosphatidylethanolamine (lyso-PE), producing more cellular PE-MUFAs and therefore reducing cellular PE-PUFAs (polyunsaturated fatty acids). MBOAT2 suppresses ferroptosis through phospholipid remodeling. In addition, MBOAT2 expression is upregulated by androgen receptor (AR) signaling in AR prostate cancer cells, therefore inhibiting their ferroptosis (3). Alternate Names 1-acylglycerophosphate O-acyltransferase; 1-acylglycerophosphoethanolamine O-acyltransferase; FLJ14415; FLJ90298; LPAAT; LPCAT4; LPEAT; LPLAT 2; Lyso-PA acyltransferase; Lyso-PE acyltransferase; Lysophosphatidic acid acyltransferase; Lysophosphatidylethanolamine acyltransferase; Lysophospholipid acyltransferase 2; MBOA2; MBOAT2; membrane bound O-acyltransferase domain containing 2; Membrane-bound O-acyltransferase domain-containing protein 2; O-acyltransferase (membrane bound) domain containing 2; O-acyltransferase domain-containing protein 2; OACT2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG