CST, 71747S, Integrin alpha1/CD49a Antibody

Polyclonal Antibody for studying ITGA1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Integrin alpha1/CD49a Antibody (CST #71747) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Integrin alpha1/CD49a Antibody recognizes endogenous levels of total integrin α1/CD49a protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu794 of human integrin α1/CD49a protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Integrin α1 (CD49a, VLA-1, ITGA1) pairs with integrin β1 to form an α1β1 dimer on the cell surface. This integrin dimer plays important roles in colorectal cancer and pancreatic cancer cell proliferation, migration, and metastasis (4-6). The cytoplasmic tail of integrin α1 activates ERK and FAK/Src signaling to promote these biological processes (7,8). In immune systems, integrin α1 expression defines a tissue-resident cytotoxic T cell population contributing to both cancer immunity and vitiligo, an autoimmune disease (9,10). Alternate Names CD49 antigen-like family member A; CD49a; Integrin alpha-1; integrin subunit alpha 1; integrin, alpha 1; ITA1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200 SOURCE: Rabbit