CST, 71756T, Glu-Glu Tag (F3Z5J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Glu-Glu Tag (F3Z5J) Rabbit Monoclonal Antibody (CST #71756) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Glu-Glu Tag (F3Z5J) Rabbit Monoclonal Antibody detects recombinant proteins containing the Glu-Glu epitope tag. This antibody recognizes the Glu-Glu sequence fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed Glu-Glu-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect a band of unknown origin at 30 kDa in some rodent cell lines. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic Glu-Glu tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG