CST, 71810S, CRBN (D8H3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CRBN. Validated for WB,WB,IP,IHC. Available in 3 sizes. Highly specific and rigorously validated in-house, CRBN (D8H3S) Rabbit Monoclonal Antibody (CST #71810) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 46351 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity CRBN (D8H3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRBN protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro44 of human CRBN protein. Background CRBN/Cereblon, a substrate receptor for the CRL4 E3 ubiquitin ligase, was originally identified for its role in autosomal recessive nonsyndromic mental retardation (1). CRBN's role in the regulation of the AMPK pathway has been linked to various human diseases, including cardiovascular disease and obesity, and its involvement in proteasomal degradation may be important in neuropsychiatric diseases (2). CRBN has also been shown to enhance the effects of immunomodulatory imide drugs (IMiDs) in multiple myeloma, and may be a predictive marker for therapy (2-4). Alternate Names cereblon; CRBN; DKFZp781K0715; MGC27358; MRT2; MRT2A; Protein cereblon; protein x 0001 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG