CST, 71812S, TMEM115 (F6N4Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TMEM115. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM115 (F6N4Y) Rabbit Monoclonal Antibody (CST #71812) is ready to ship. Product Usage Information This product was developed by CST ® scientists in collaboration with The Michael J. Fox Foundation (MJFF). Our partnership aims to accelerate Parkinson's disease (PD) research by identifying and developing high-quality monoclonal antibodies for promising PD targets. Visit the CST Parkinson's Disease Resource Center to learn more: https://cst-science.com/PD-Resources . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TMEM115 (F6N4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total TMEM115 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp325 of human TMEM115 protein. Background Transmembrane protein 115 (TMEM115) is a 35 kDa protein localized to the Golgi cisternae. Both the N- and C-terminal ends of TMEM115 are oriented toward the cytosol, with four transmembrane domains and a Golgi targeting sequence located in between. While the functions of this protein are not fully understood, it is suggested to play roles in Golgi-to-ER retrograde transport and glycosylation (1). Elevated TMEM115 expression levels have been observed in glioma and hepatocellular carcinoma, indicating its potential as a biomarker and therapeutic target for these cancers (2,3). Golgi dysfunction is associated with many diseases, including cancer, neurodegeneration, and cardiovascular diseases. As a Golgi-specific transmembrane protein, TMEM115 can be leveraged for immunoprecipitation experiments to purify and study the Golgi proteome, metabolome, and lipidome in health and disease (4). Alternate Names PL6; Placental protein 6; PP6; Protein PL6; testicular tissue protein Li 201; TM115; TMEM115; Transmembrane protein 115 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG