CST, 71820T, Podoplanin (F9S2C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Podoplanin mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Podoplanin (F9S2C) Rabbit Monoclonal Antibody (CST #71820) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:800 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Podoplanin (F9S2C) Rabbit Monoclonal Antibody recognizes endogenous levels of total Podoplanin protein. Non-specific staining of fixed frozen mouse liver may be observed by immunofluorescence. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp30 of mouse podoplanin protein. Background Podoplanin (aggrus, glycoprotein 36) is a single-pass transmembrane protein belonging to the type-1 family of sialomucin-like glycoproteins. Podoplanin was first described in the rat as a surface glycoprotein that regulated podocyte morphology (1). It is now commonly used as a marker of lymphatic endothelial cells, where its expression is associated with the process of lymphangiogenesis (2). Its role in this regard is presumably due to its putative involvement in regulating actin cytoskeleton dynamics (3). Research studies have shown that podoplanin expression is upregulated in a number of tumor types including colorectal cancers (4), oral squamous cell carcinomas (5), and germ cell tumors (6), with higher expression levels often associated with more aggressive tumors (7). Research studies have suggested a functional role for podoplanin in the stromal microenvironment of tumors. For example, it has been reported that podoplanin expression in cancer-associated fibroblasts (CAFs) is positively associated with a stromal environment that promotes cancer progression (8,9). Alternate Names Aggrus; E11; Glycoprotein 38; Gp3; Gp38; OTS; OTS-8; Ots8; OTTMUSP00000010796; PA2.; PA2.26; PA2.26 antigen; Pdpn; Podoplanin; RAND; RANDAM-2; T1-alpha; T1A; T1alpha; Transmembrane glycoprotein E11 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG