CST, 71835S, K-Ras (E2M9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KRas. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, K-Ras (E2M9G) Rabbit Monoclonal Antibody (CST #71835) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity K-Ras (E2M9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total K-Ras protein. This antibody cross-reacts with N-Ras in a K-Ras preferred manner, but does not cross-react with H-Ras or R-Ras protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val125 of human K-Ras protein. Background The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). Alternate Names 'C-K-RAS; c-K-ras; c-Ki-ras; c-Ki-ras2; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; CFC2; GTPase KRas; GTPase KRas, N-terminally processed; K-Ras; K-Ras 2; K-ras p21 protein; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; Ki-Ras; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; KRAS; KRAS proto-oncogene, GTPase; KRAS1; KRAS2; NS; NS3; OES; oncogene KRAS2; PR310 c-K-ras oncogene; RALD; RASK; RASK2; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG