CST, 71873S, FTSJ3 Antibody

Polyclonal Antibody for studying FTSJ3. Validated for Western Blotting. Highly specific and rigorously validated in-house, FTSJ3 Antibody (CST #71873) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity FTSJ3 Antibody recognizes endogenous levels of total FTSJ3 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile651 of human FTSJ3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background FTSJ3 is an RNA 2'-O-methyltransferase originally discovered through its role in processing of 34S pre-rRNA (1). In addition to contributing to RNA stabilization, 2'-O-methylation at the 5'-cap enables the innate immune system to distinguish self from non-self mRNA (2). While some viruses encode their own 2'-O-methyltransferase enabling them to avoid detection by the innate immune system, HIV does not (3,4). However, it was recently demonstrated that FTSJ3 is recruited to HIV RNA resulting in 2'-O-methylation of the HIV RNA genome and evasion of immune detection (5). Cells deficient in FTSJ3 produced virus with less 2'-O-methylation and upregulated type I interferon expression leading to reduced HIV expression and replication (5). Alternate Names 2'-O-ribose RNA methyltransferase SPB1 homolog; EPCS3; FLJ20062; FtsJ homolog 3; FtsJ homolog 3 (E. coli); FtsJ RNA 2'-O-methyltransferase 3; FtsJ RNA methyltransferase homolog 3; FTSJ3; pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3; pre-rRNA processing protein FTSJ3; Protein ftsJ homolog 3; Putative rRNA methyltransferase 3; RRMJ3; rRNA (uridine-2'-O-)-methyltransferase 3; SPB1; SPB1 RNA methyltransferase homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit