CST, 72031S, CD57 (HNK-1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for IHC Leica Bond,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CD57 (HNK-1) Mouse Monoclonal Antibody (CST #72031) is ready to ship. Product Usage Information IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present, but will not interfere with antibody performance. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD57 (HNK-1) Mouse Monoclonal Antibody recognizes the CD57 carbohydrate modification produced by the B3GAT1 protein enzyme. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells. Background CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8 T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1). Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgM