CST, 72033SF, TFF1/pS2 (D2Y1J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TFF1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TFF1/pS2 (D2Y1J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #72033) is ready to ship. Product Usage Information This product is the carrier free version of product #15571. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15571 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TFF1/pS2 (D2Y1J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TFF1/pS2 protein. This antibody does not cross-react with either TFF2 or TFF3 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human TFF1/pS2 protein. Background The trefoil factor (TFF) family of proteins (TFF1/pS2, TFF2, and TFF3) are a group of highly conserved, secreted polypeptides that are expressed by mucus-secreting cells of the gastrointestinal tract. Within the gastrointestinal tract, TFFs display both common and distinct expression patterns (1). Collectively, the TFF family of proteins play a prominant role in the protection and repair of the mucous epithelia lining the gastrointestinal tract through their interactions with mucins (2). TFFs have been shown to regulate a number of cellular processes such as migration, apoptosis, and proliferation. In humans, dysregulated expression of TFFs has been observed in inflammatory bowel diseases as well as tumors of the breast, colon, lung, and stomach (2). TFF1/pS2 is a gastric peptide that is highly expressed by mucosal goblet cells of the stomach, where it is thought to play a role in maintaining the integrity of the epithelial layer of the mucosa through the regulation of cell-cell adhesion and cell migration (2,3). Research studies have shown that TFF1 functions as a tumor suppressor in the stomach as its expression is frequently lost in gastric carcinomas, largely due to promoter hypermethylation (4-8). Research studies have also demonstrated that is a transcriptional target of estrogen receptor-α and that TFF1 expression in breast carcinoma may be used as a predictive biomarker for response to anti-estrogen therapy (9,10). Alternate Names BCEI; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; HP1.A; HPS2; P04155; pNR-2; Polypeptide P1.A; Protein pS2; pS2; TFF1; Trefoil factor 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG