CST, 72046S, Tox2 Antibody

Polyclonal Antibody for studying Tox2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Tox2 Antibody (CST #72046) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tox2 Antibody recognizes endogenous levels of total Tox2 protein. This antibody does not cross-react with Tox, Tox3, or Tox4 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu343 of human Tox2 protein. Antibodies are purified by peptide affinity chromatography. Background Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3 T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14). Alternate Names C20orf100; dJ1108D11.2; dJ495O3.1; GCX-1; GCX1; granulosa cell HMG box 1; Granulosa cell HMG box protein 1; granulosa cell HMG-box protein 1; MGC15880; TOX high mobility group box family member 2; TOX2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 SOURCE: Rabbit