CST, 72099S, LIME1 (E3M3B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LIME1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, LIME1 (E3M3B) Rabbit Monoclonal Antibody (CST #72099) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LIME1 (E3M3B) Rabbit Monoclonal Antibody recognizes endogenous levels of total LIME1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro211 of human LIME1 protein. Background Lck-interacting transmembrane adaptor 1 (LIME1) is a type III transmembrane adaptor protein that participates in lymphocyte signaling and is predominantly expressed in T lineage cells and plasma cells (1,2). LIME1 also harbors a palmitoylation motif for targeting to lipid rafts and potential tyrosine phosphorylation sites that are engaged upon CD4 and CD8 cross-linking (1). Research studies have demonstrated that in response to T cell receptor (TCR) engagement, LIME1 expression is upregulated, undergoes tyrosine phosphorylation, and associates with numerous signaling proteins such as Lck, GADS, and Vav. Collectively, these observations point toward a role for LIME1 in the regulation of T cell activation and adhesion (3,4). Alternate Names dJ583P15.4; FLJ20406; Lck interacting transmembrane adaptor 1; Lck-interacting membrane protein; Lck-interacting molecule; Lck-interacting transmembrane adapter 1; LIME; LIME1; LP8067 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG