CST, 72219S, Lloviu Virus VP35 Antibody

Polyclonal Antibody for studying Polymerase cofactor VP35 cuevavirus (LLOV). Validated for Western Blotting. Highly specific and rigorously validated in-house, Lloviu Virus VP35 Antibody (CST #72219) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Lloviu Virus VP35 Antibody recognizes endogenous levels of total Lloviu virus VP35 protein. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro47 of Lloviu virus VP35 protein. Antibodies are purified by peptide affinity chromatography. Background The Lloviu virus (LLOV) is a pathogenic filovirus identified in bat populations in southern Europe (1-3). It belongs to the Filoviridae family, members of which (e.g., Ebola, Marburg) are known to cause fatal hemorrhagic diseases in primates, including humans (4). Transcription and replication of filoviruses requires formation of a ribonucleoprotein (RNP) complex, composed of nucleoprotein (NP) together with viral polymerase enzymes and cofactors. Viral Protein 35 (VP35), a polymerase cofactor, is one of the key components of the RNP complex in filoviruses (5). In addition to its role in promoting viral replication, research studies in both bat and human cells have shown that Lloviu virus VP35 can suppress the host innate immune response to viral infection, in part through suppression of the interferon response (6). These immunosuppressive functions of Lloviu virus VP35 are consistent with those observed for VP35 homologs in other filovirus species (7,8). Alternate Names polymerase complex protein; VP35 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit